6-Pyruvoyl tetrahydropterin synthase, an enzyme with a novel type of active site involving both zinc binding and an intersubunit catalytic triad motif; site-directed mutagenesis of the proposed active center, characterization of the metal binding site and modelling of substrate binding. [electronic resource]
Producer: 19951122Description: 358-69 p. digitalISSN:- 0022-2836
- Alcohol Oxidoreductases -- chemistry
- Amino Acid Sequence
- Animals
- Apoenzymes -- chemistry
- Base Sequence
- Binding Sites
- DNA Primers
- Escherichia coli
- Humans
- Macromolecular Substances
- Magnesium -- analysis
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phosphorus-Oxygen Lyases
- Point Mutation
- Protein Structure, Secondary
- Rats
- Recombinant Fusion Proteins -- biosynthesis
- Recombinant Proteins -- chemistry
- Salmon
- Sequence Homology, Amino Acid
- Spectrophotometry, Atomic
- Zinc -- metabolism
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Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
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