Identifying novel protein complexes in cancer cells using epitope-tagging of endogenous human genes and affinity-purification mass spectrometry. [electronic resource]
Producer: 20130523Description: 5630-41 p. digitalISSN:- 1535-3907
- Adaptor Proteins, Signal Transducing -- isolation & purification
- Cell Nucleus -- metabolism
- Chromatography, Affinity -- methods
- Colonic Neoplasms -- metabolism
- Cytoplasm -- metabolism
- Dependovirus
- Epitope Mapping -- methods
- Genetic Vectors
- Genome, Human
- HCT116 Cells
- HEK293 Cells
- Humans
- Mass Spectrometry -- methods
- Microfilament Proteins -- isolation & purification
- Multiprotein Complexes -- isolation & purification
- Neoplasm Proteins -- isolation & purification
- Protein Interaction Mapping -- methods
- Proteomics -- methods
- Receptors, Cytoplasmic and Nuclear -- isolation & purification
- Reproducibility of Results
- Sensitivity and Specificity
- Trans-Activators
- Transfection
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Publication Type: Journal Article; Research Support, N.I.H., Extramural
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