Identifying novel protein complexes in cancer cells using epitope-tagging of endogenous human genes and affinity-purification mass spectrometry.

Song, Jing

Identifying novel protein complexes in cancer cells using epitope-tagging of endogenous human genes and affinity-purification mass spectrometry. [electronic resource] - Journal of proteome research Dec 2012 - 5630-41 p. digital

Publication Type: Journal Article; Research Support, N.I.H., Extramural

1535-3907

10.1021/pr300598t doi


Adaptor Proteins, Signal Transducing--isolation & purification
Cell Nucleus--metabolism
Chromatography, Affinity--methods
Colonic Neoplasms--metabolism
Cytoplasm--metabolism
Dependovirus
Epitope Mapping--methods
Genetic Vectors
Genome, Human
HCT116 Cells
HEK293 Cells
Humans
Mass Spectrometry--methods
Microfilament Proteins--isolation & purification
Multiprotein Complexes--isolation & purification
Neoplasm Proteins--isolation & purification
Protein Interaction Mapping--methods
Proteomics--methods
Receptors, Cytoplasmic and Nuclear--isolation & purification
Reproducibility of Results
Sensitivity and Specificity
Trans-Activators
Transfection