A high-throughput FRET-based assay for determination of Atg4 activity. [electronic resource]
Producer: 20130103Description: 401-12 p. digitalISSN:- 1554-8635
- Adaptor Proteins, Signal Transducing
- Amino Acid Sequence
- Autophagy-Related Protein 8 Family
- Autophagy-Related Proteins
- Cell Extracts
- Cysteine Endopeptidases -- metabolism
- Electrophoresis, Polyacrylamide Gel
- Fluorescence Resonance Energy Transfer -- methods
- HEK293 Cells
- High-Throughput Screening Assays -- methods
- Humans
- Kinetics
- Microfilament Proteins
- Molecular Sequence Data
- Protease Inhibitors -- pharmacology
- Recombinant Fusion Proteins -- metabolism
- Reproducibility of Results
- Substrate Specificity -- drug effects
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Publication Type: Journal Article; Research Support, N.I.H., Extramural
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