A high-throughput FRET-based assay for determination of Atg4 activity.

Li, Min

A high-throughput FRET-based assay for determination of Atg4 activity. [electronic resource] - Autophagy Mar 2012 - 401-12 p. digital

Publication Type: Journal Article; Research Support, N.I.H., Extramural

1554-8635

10.4161/auto.18777 doi


Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Autophagy-Related Protein 8 Family
Autophagy-Related Proteins
Cell Extracts
Cysteine Endopeptidases--metabolism
Electrophoresis, Polyacrylamide Gel
Fluorescence Resonance Energy Transfer--methods
HEK293 Cells
High-Throughput Screening Assays--methods
Humans
Kinetics
Microfilament Proteins
Molecular Sequence Data
Protease Inhibitors--pharmacology
Recombinant Fusion Proteins--metabolism
Reproducibility of Results
Substrate Specificity--drug effects