Development of high-throughput TR-FRET and AlphaScreen assays for identification of potent inhibitors of PDK1.
Xu, Zangwei
Development of high-throughput TR-FRET and AlphaScreen assays for identification of potent inhibitors of PDK1. [electronic resource] - Journal of biomolecular screening Dec 2009 - 1257-62 p. digital
Publication Type: Journal Article
1552-454X
10.1177/1087057109349356 doi
3-Phosphoinositide-Dependent Protein Kinases
Adenosine Triphosphate--metabolism
Biotinylation--drug effects
Fluorescence Resonance Energy Transfer--methods
High-Throughput Screening Assays--methods
Humans
Kinetics
Peptides--metabolism
Protein Kinase Inhibitors--analysis
Protein Serine-Threonine Kinases--antagonists & inhibitors
Proto-Oncogene Proteins c-akt--metabolism
Reproducibility of Results
Signal Transduction--drug effects
Structure-Activity Relationship
Substrate Specificity--drug effects
Time Factors
Development of high-throughput TR-FRET and AlphaScreen assays for identification of potent inhibitors of PDK1. [electronic resource] - Journal of biomolecular screening Dec 2009 - 1257-62 p. digital
Publication Type: Journal Article
1552-454X
10.1177/1087057109349356 doi
3-Phosphoinositide-Dependent Protein Kinases
Adenosine Triphosphate--metabolism
Biotinylation--drug effects
Fluorescence Resonance Energy Transfer--methods
High-Throughput Screening Assays--methods
Humans
Kinetics
Peptides--metabolism
Protein Kinase Inhibitors--analysis
Protein Serine-Threonine Kinases--antagonists & inhibitors
Proto-Oncogene Proteins c-akt--metabolism
Reproducibility of Results
Signal Transduction--drug effects
Structure-Activity Relationship
Substrate Specificity--drug effects
Time Factors