Development and full validation of six inhibition assays for five major cytochrome P450 enzymes in human liver microsomes using an automated 96-well microplate incubation format and LC-MS/MS analysis.

Yao, Ming

Development and full validation of six inhibition assays for five major cytochrome P450 enzymes in human liver microsomes using an automated 96-well microplate incubation format and LC-MS/MS analysis. [electronic resource] - Journal of pharmaceutical and biomedical analysis May 2007 - 211-23 p. digital

Publication Type: Journal Article

0731-7085

10.1016/j.jpba.2007.02.034 doi


Aryl Hydrocarbon Hydroxylases--antagonists & inhibitors
Autoanalysis
Biological Assay
Chromatography, Liquid--methods
Cytochrome P-450 CYP1A2--chemistry
Cytochrome P-450 CYP1A2 Inhibitors
Cytochrome P-450 CYP2C19
Cytochrome P-450 CYP2C9
Cytochrome P-450 CYP2D6--chemistry
Cytochrome P-450 CYP2D6 Inhibitors
Cytochrome P-450 CYP3A
Cytochrome P-450 Enzyme Inhibitors
Cytochrome P-450 Enzyme System--chemistry
Enzyme Inhibitors--pharmacology
Humans
Inhibitory Concentration 50
Isoenzymes--antagonists & inhibitors
Kinetics
Microsomes, Liver--enzymology
Mixed Function Oxygenases--antagonists & inhibitors
Molecular Structure
Reproducibility of Results
Substrate Specificity
Tandem Mass Spectrometry--methods