000 01971 a2200601 4500
005 20250513100658.0
264 0 _c19961029
008 199610s 0 0 eng d
022 _a0006-2960
024 7 _a10.1021/bi960234o
_2doi
040 _aNLM
_beng
_cNLM
100 1 _aHuang, B
245 0 0 _aPhospholipase A2 engineering. Deletion of the C-terminus segment changes substrate specificity and uncouples calcium and substrate binding at the zwitterionic interface.
_h[electronic resource]
260 _bBiochemistry
_cSep 1996
300 _a12164-74 p.
_bdigital
500 _aPublication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
650 0 4 _aAmino Acid Sequence
650 0 4 _aAnimals
650 0 4 _aBinding Sites
650 0 4 _aCalcium
_xmetabolism
650 0 4 _aCalorimetry
650 0 4 _aCattle
650 0 4 _aCloning, Molecular
650 0 4 _aComputer Graphics
650 0 4 _aDNA Primers
650 0 4 _aEnzyme Stability
650 0 4 _aEscherichia coli
650 0 4 _aMagnetic Resonance Spectroscopy
650 0 4 _aModels, Molecular
650 0 4 _aMutagenesis, Site-Directed
650 0 4 _aPancreas
_xenzymology
650 0 4 _aPhospholipases A
_xbiosynthesis
650 0 4 _aPhospholipases A2
650 0 4 _aPoint Mutation
650 0 4 _aProtein Conformation
650 0 4 _aProtein Denaturation
650 0 4 _aProtein Engineering
650 0 4 _aRecombinant Proteins
_xbiosynthesis
650 0 4 _aSensitivity and Specificity
650 0 4 _aSequence Deletion
650 0 4 _aSubstrate Specificity
650 0 4 _aThermodynamics
700 1 _aYu, B Z
700 1 _aRogers, J
700 1 _aByeon, I J
700 1 _aSekar, K
700 1 _aChen, X
700 1 _aSundaralingam, M
700 1 _aTsai, M D
700 1 _aJain, M K
773 0 _tBiochemistry
_gvol. 35
_gno. 37
_gp. 12164-74
856 4 0 _uhttps://doi.org/10.1021/bi960234o
_zAvailable from publisher's website
999 _c8805470
_d8805470