000 01329 a2200361 4500
005 20250512045403.0
264 0 _c19880315
008 198803s 0 0 eng d
022 _a0076-6879
024 7 _a10.1016/0076-6879(87)54083-6
_2doi
040 _aNLM
_beng
_cNLM
100 1 _aZoller, M J
245 0 0 _aOligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template.
_h[electronic resource]
260 _bMethods in enzymology
_c1987
300 _a329-50 p.
_bdigital
500 _aPublication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
650 0 4 _aBase Sequence
650 0 4 _aCloning, Molecular
650 0 4 _aColiphages
_xgenetics
650 0 4 _aDNA, Single-Stranded
_xgenetics
650 0 4 _aDNA, Viral
_xgenetics
650 0 4 _aEscherichia coli
_xgenetics
650 0 4 _aGenes, Synthetic
650 0 4 _aGenetic Engineering
_xmethods
650 0 4 _aGenetic Vectors
650 0 4 _aMutation
650 0 4 _aNucleic Acid Hybridization
650 0 4 _aOligodeoxyribonucleotides
650 0 4 _aTemplates, Genetic
700 1 _aSmith, M
773 0 _tMethods in enzymology
_gvol. 154
_gp. 329-50
856 4 0 _uhttps://doi.org/10.1016/0076-6879(87)54083-6
_zAvailable from publisher's website
999 _c3329564
_d3329564