000 01546 a2200421 4500
005 20250517020025.0
264 0 _c20160322
008 201603s 0 0 eng d
022 _a1096-0279
024 7 _a10.1016/j.pep.2015.02.010
_2doi
040 _aNLM
_beng
_cNLM
100 1 _aLiu, Xinxin
245 0 0 _aUse of adenylate kinase as a solubility tag for high level expression of T4 DNA ligase in Escherichia coli.
_h[electronic resource]
260 _bProtein expression and purification
_cMay 2015
300 _a79-84 p.
_bdigital
500 _aPublication Type: Journal Article; Research Support, Non-U.S. Gov't
650 0 4 _aAdenosine Diphosphate
_xmetabolism
650 0 4 _aAdenosine Triphosphate
_xmetabolism
650 0 4 _aAdenylate Kinase
_xisolation & purification
650 0 4 _aChromatography, Affinity
650 0 4 _aCloning, Molecular
650 0 4 _aDNA Ligases
_xisolation & purification
650 0 4 _aElectrophoresis, Polyacrylamide Gel
650 0 4 _aEnzyme Assays
650 0 4 _aEscherichia coli
_xmetabolism
650 0 4 _aGene Expression
650 0 4 _aGenetic Vectors
_xmetabolism
650 0 4 _aRecombinant Fusion Proteins
_xisolation & purification
650 0 4 _aSolubility
700 1 _aHuang, Anliang
700 1 _aLuo, Dan
700 1 _aLiu, Haipeng
700 1 _aHan, Huzi
700 1 _aXu, Yang
700 1 _aLiang, Peng
773 0 _tProtein expression and purification
_gvol. 109
_gp. 79-84
856 4 0 _uhttps://doi.org/10.1016/j.pep.2015.02.010
_zAvailable from publisher's website
999 _c24641337
_d24641337