000 01760 a2200505 4500
005 20250517014018.0
264 0 _c20151007
008 201510s 0 0 eng d
022 _a1940-6029
024 7 _a10.1007/978-1-4939-2398-4_4
_2doi
040 _aNLM
_beng
_cNLM
100 1 _aMori, Hirotada
245 0 0 _aIdentification of essential genes and synthetic lethal gene combinations in Escherichia coli K-12.
_h[electronic resource]
260 _bMethods in molecular biology (Clifton, N.J.)
_c2015
300 _a45-65 p.
_bdigital
500 _aPublication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
650 0 4 _aAmino Acid Sequence
650 0 4 _aBase Sequence
650 0 4 _aConjugation, Genetic
650 0 4 _aDNA Primers
_xmetabolism
650 0 4 _aElectroporation
650 0 4 _aEscherichia coli K12
_xgenetics
650 0 4 _aGene Knockout Techniques
650 0 4 _aGenes, Bacterial
650 0 4 _aGenes, Essential
650 0 4 _aGenes, Lethal
650 0 4 _aGenes, Synthetic
650 0 4 _aGenetic Complementation Test
650 0 4 _aGenomics
_xmethods
650 0 4 _aMolecular Sequence Data
650 0 4 _aMutation
650 0 4 _aPolymerase Chain Reaction
650 0 4 _aReproducibility of Results
650 0 4 _aTransformation, Genetic
700 1 _aBaba, Tomoya
700 1 _aYokoyama, Katsushi
700 1 _aTakeuchi, Rikiya
700 1 _aNomura, Wataru
700 1 _aMakishi, Kazuichi
700 1 _aOtsuka, Yuta
700 1 _aDose, Hitomi
700 1 _aWanner, Barry L
773 0 _tMethods in molecular biology (Clifton, N.J.)
_gvol. 1279
_gp. 45-65
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-2398-4_4
_zAvailable from publisher's website
999 _c24580045
_d24580045