000 01242 a2200313 4500
005 20250516204137.0
264 0 _c20150109
008 201501s 0 0 eng d
022 _a1096-0279
024 7 _a10.1016/j.pep.2014.03.008
_2doi
040 _aNLM
_beng
_cNLM
100 1 _aCamilo, Cesar M
245 0 0 _aHigh-throughput cloning, expression and purification of glycoside hydrolases using Ligation-Independent Cloning (LIC).
_h[electronic resource]
260 _bProtein expression and purification
_cJul 2014
300 _a35-42 p.
_bdigital
500 _aPublication Type: Journal Article; Research Support, Non-U.S. Gov't
650 0 4 _aCloning, Molecular
_xmethods
650 0 4 _aDNA-Directed DNA Polymerase
_xmetabolism
650 0 4 _aEscherichia coli
_xenzymology
650 0 4 _aGenetic Vectors
650 0 4 _aGlycoside Hydrolases
_xbiosynthesis
650 0 4 _aHigh-Throughput Nucleotide Sequencing
650 0 4 _aRecombinant Fusion Proteins
_xgenetics
650 0 4 _aSolubility
650 0 4 _aThioredoxins
_xchemistry
700 1 _aPolikarpov, Igor
773 0 _tProtein expression and purification
_gvol. 99
_gp. 35-42
856 4 0 _uhttps://doi.org/10.1016/j.pep.2014.03.008
_zAvailable from publisher's website
999 _c23687867
_d23687867