000 01589 a2200433 4500
005 20250516190025.0
264 0 _c20141008
008 201410s 0 0 eng d
022 _a1932-6203
024 7 _a10.1371/journal.pone.0082538
_2doi
040 _aNLM
_beng
_cNLM
100 1 _aImamura, Morikazu
245 0 0 _aInsect cell-derived cofactors become fully functional after proteinase K and heat treatment for high-fidelity amplification of glycosylphosphatidylinositol-anchored recombinant scrapie and BSE prion proteins.
_h[electronic resource]
260 _bPloS one
_c2013
300 _ae82538 p.
_bdigital
500 _aPublication Type: Journal Article; Research Support, Non-U.S. Gov't
650 0 4 _aAnimals
650 0 4 _aBaculoviridae
_xgenetics
650 0 4 _aBiological Assay
650 0 4 _aBlotting, Western
650 0 4 _aEndopeptidase K
_xmetabolism
650 0 4 _aGlycosylphosphatidylinositols
_xchemistry
650 0 4 _aHot Temperature
650 0 4 _aPolymerase Chain Reaction
650 0 4 _aPrPC Proteins
_xmetabolism
650 0 4 _aPrPSc Proteins
_xmetabolism
650 0 4 _aPrions
_xmetabolism
650 0 4 _aRecombinant Proteins
_xgenetics
700 1 _aKato, Nobuko
700 1 _aOkada, Hiroyuki
700 1 _aYoshioka, Miyako
700 1 _aIwamaru, Yoshifumi
700 1 _aShimizu, Yoshihisa
700 1 _aMohri, Shirou
700 1 _aYokoyama, Takashi
700 1 _aMurayama, Yuichi
773 0 _tPloS one
_gvol. 8
_gno. 12
_gp. e82538
856 4 0 _uhttps://doi.org/10.1371/journal.pone.0082538
_zAvailable from publisher's website
999 _c23394989
_d23394989