000 01101 a2200313 4500
005 20250516150257.0
264 0 _c20140224
008 201402s 0 0 eng d
022 _a1559-0305
024 7 _a10.1007/s12033-013-9665-5
_2doi
040 _aNLM
_beng
_cNLM
100 1 _aBu, Yuling
245 0 0 _aLong distance multiple-site directed plasmid mutagenesis by one-step PCR using non-overlapped primers.
_h[electronic resource]
260 _bMolecular biotechnology
_cSep 2013
300 _a49-53 p.
_bdigital
500 _aPublication Type: Journal Article; Research Support, Non-U.S. Gov't
650 0 4 _aDNA Primers
_xchemistry
650 0 4 _aMutagenesis, Site-Directed
_xmethods
650 0 4 _aPlasmids
650 0 4 _aPolymerase Chain Reaction
_xmethods
650 0 4 _aTemperature
700 1 _aWang, Hui
700 1 _aLi, Jing
700 1 _aZhang, Zhaoxin
700 1 _aHu, Yinghe
700 1 _aXu, Zhiliang
773 0 _tMolecular biotechnology
_gvol. 55
_gno. 1
_gp. 49-53
856 4 0 _uhttps://doi.org/10.1007/s12033-013-9665-5
_zAvailable from publisher's website
999 _c22725181
_d22725181