000 | 01581 a2200445 4500 | ||
---|---|---|---|
005 | 20250514233257.0 | ||
264 | 0 | _c20050725 | |
008 | 200507s 0 0 eng d | ||
022 | _a1362-4962 | ||
024 | 7 |
_a10.1093/nar/gni103 _2doi |
|
040 |
_aNLM _beng _cNLM |
||
100 | 1 | _aSuzuki, Yasuhiro | |
245 | 0 | 0 |
_aA novel high-throughput (HTP) cloning strategy for site-directed designed chimeragenesis and mutation using the Gateway cloning system. _h[electronic resource] |
260 |
_bNucleic acids research _cJul 2005 |
||
300 |
_ae109 p. _bdigital |
||
500 | _aPublication Type: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't | ||
650 | 0 | 4 |
_aCloning, Molecular _xmethods |
650 | 0 | 4 | _aDNA Shuffling |
650 | 0 | 4 |
_aDNA, Circular _xchemistry |
650 | 0 | 4 |
_aEscherichia coli _xgenetics |
650 | 0 | 4 | _aGenetic Vectors |
650 | 0 | 4 | _aMutagenesis, Site-Directed |
650 | 0 | 4 |
_aPlasmids _xchemistry |
650 | 0 | 4 | _aPolymerase Chain Reaction |
650 | 0 | 4 |
_aRecombinant Fusion Proteins _xgenetics |
650 | 0 | 4 | _aRecombination, Genetic |
650 | 0 | 4 | _aSequence Homology, Nucleic Acid |
650 | 0 | 4 | _aTransformation, Bacterial |
700 | 1 | _aKagawa, Naoko | |
700 | 1 | _aFujino, Toru | |
700 | 1 | _aSumiya, Tsuyoshi | |
700 | 1 | _aAndoh, Taichi | |
700 | 1 | _aIshikawa, Kumiko | |
700 | 1 | _aKimura, Rie | |
700 | 1 | _aKemmochi, Kiyokazu | |
700 | 1 | _aOhta, Tsutomu | |
700 | 1 | _aTanaka, Shigeo | |
773 | 0 |
_tNucleic acids research _gvol. 33 _gno. 12 _gp. e109 |
|
856 | 4 | 0 |
_uhttps://doi.org/10.1093/nar/gni103 _zAvailable from publisher's website |
999 |
_c15665806 _d15665806 |