000 01205 a2200337 4500
005 20250511180643.0
264 0 _c19930208
008 199302s 0 0 eng d
022 _a1054-9803
024 7 _a10.1101/gr.1.4.277
_2doi
040 _aNLM
_beng
_cNLM
100 1 _aShankarappa, B
245 0 0 _aIntroduction of multiple restriction enzyme sites by in vitro mutagenesis using the polymerase chain reaction.
_h[electronic resource]
260 _bPCR methods and applications
_cMay 1992
300 _a277-8 p.
_bdigital
500 _aPublication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
650 0 4 _aBase Sequence
650 0 4 _aDNA
_xchemistry
650 0 4 _aDNA Restriction Enzymes
650 0 4 _aGenes, env
650 0 4 _aHIV-1
_xgenetics
650 0 4 _aMolecular Sequence Data
650 0 4 _aMutagenesis
650 0 4 _aPolymerase Chain Reaction
650 0 4 _aSubstrate Specificity
700 1 _aBalachandran, R
700 1 _aGupta, P
700 1 _aEhrlich, G D
773 0 _tPCR methods and applications
_gvol. 1
_gno. 4
_gp. 277-8
856 4 0 _uhttps://doi.org/10.1101/gr.1.4.277
_zAvailable from publisher's website
999 _c1342336
_d1342336