Production of soluble ScFvs with C-terminal-free thiol for site-specific conjugation or stable dimeric ScFvs on demand. [electronic resource]
Producer: 20040309Description: 16-26 p. digitalISSN:- 1043-1802
- Antibodies, Monoclonal -- chemistry
- Cell Line, Tumor
- Cloning, Molecular
- Cysteine -- chemistry
- Electrophoresis, Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- Escherichia coli -- genetics
- Female
- Fermentation
- Genetic Vectors
- Humans
- Immunoglobulin Fragments -- chemistry
- Immunoglobulin Variable Region -- biosynthesis
- Immunohistochemistry
- Indicators and Reagents
- Mutagenesis
- Peptide Library
- Polyethylene Glycols
- Recombinant Proteins -- biosynthesis
- Reducing Agents -- chemistry
- Sulfhydryl Compounds -- chemistry
- Sulfhydryl Reagents
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Publication Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.
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