TY  - GEN
AU  - Huang,B
AU  - Yu,B Z
AU  - Rogers,J
AU  - Byeon,I J
AU  - Sekar,K
AU  - Chen,X
AU  - Sundaralingam,M
AU  - Tsai,M D
AU  - Jain,M K
TI  - Phospholipase A2 engineering. Deletion of the C-terminus segment changes substrate specificity and uncouples calcium and substrate binding at the zwitterionic interface
SN  - 0006-2960
PY  - 1996///1029
KW  - Amino Acid Sequence
KW  - Animals
KW  - Binding Sites
KW  - Calcium
KW  - metabolism
KW  - Calorimetry
KW  - Cattle
KW  - Cloning, Molecular
KW  - Computer Graphics
KW  - DNA Primers
KW  - Enzyme Stability
KW  - Escherichia coli
KW  - Magnetic Resonance Spectroscopy
KW  - Models, Molecular
KW  - Mutagenesis, Site-Directed
KW  - Pancreas
KW  - enzymology
KW  - Phospholipases A
KW  - biosynthesis
KW  - Phospholipases A2
KW  - Point Mutation
KW  - Protein Conformation
KW  - Protein Denaturation
KW  - Protein Engineering
KW  - Recombinant Proteins
KW  - Sensitivity and Specificity
KW  - Sequence Deletion
KW  - Substrate Specificity
KW  - Thermodynamics
N1  - Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S
UR  - https://doi.org/10.1021/bi960234o
ER  -