Huang, B <br/><br/>
Phospholipase A2 engineering. Deletion of the C-terminus segment changes substrate specificity and uncouples calcium and substrate binding at the zwitterionic interface.  [electronic resource] 

 -  Biochemistry  Sep 1996 
 - 12164-74 p.  digital 
<br/><br/> Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. 
<br/><br/><label>ISSN: </label>0006-2960 
<br/><br/><label>Standard No.: </label>10.1021/bi960234o  doi 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Amino Acid Sequence<br/>Animals<br/>Binding Sites<br/>Calcium--metabolism<br/>Calorimetry<br/>Cattle<br/>Cloning, Molecular<br/>Computer Graphics<br/>DNA Primers<br/>Enzyme Stability<br/>Escherichia coli<br/>Magnetic Resonance Spectroscopy<br/>Models, Molecular<br/>Mutagenesis, Site-Directed<br/>Pancreas--enzymology<br/>Phospholipases A--biosynthesis<br/>Phospholipases A2<br/>Point Mutation<br/>Protein Conformation<br/>Protein Denaturation<br/>Protein Engineering<br/>Recombinant Proteins--biosynthesis<br/>Sensitivity and Specificity<br/>Sequence Deletion<br/>Substrate Specificity<br/>Thermodynamics