Beyer, B M Site-directed mutagenesis of a disulfide bridge in cathepsin D: expression, activation, purification, and characterization. [electronic resource] - Advances in experimental medicine and biology 1995 - 285-7 p. digital Publication Type: Comparative Study; Journal Article ISSN: 0065-2598 Standard No.: 10.1007/978-1-4615-1871-6_33 doi Subjects--Topical Terms: Amino Acid SequenceAnimalsCathepsin D--biosynthesisCloning, MolecularCysteineDisulfidesEnzyme ActivationEscherichia coliGene ExpressionMolecular Sequence DataMutagenesis, Site-DirectedOligopeptides--metabolismPoint MutationPolymerase Chain ReactionRecombinant Proteins--biosynthesisSubstrate Specificity