Kealey, J T High-level expression and rapid purification of tRNA (m5U54)-methyltransferase. [electronic resource] - Protein expression and purification Apr 1994 - 149-52 p. digital Publication Type: Journal Article; Research Support, U.S. Gov't, P.H.S. ISSN: 1046-5928 Standard No.: 10.1006/prep.1994.1023 doi Subjects--Topical Terms: Bacterial Proteins--biosynthesisCellulose--analogs & derivativesChromatography, AffinityChromatography, DEAE-CelluloseChromatography, Ion ExchangeCloning, MolecularEnzyme InductionEscherichia coli--enzymologyGenetic VectorsRecombinant Fusion Proteins--biosynthesistRNA Methyltransferases--biosynthesis