TY  - GEN
AU  - Lewis,W S
AU  - Cody,V
AU  - Galitsky,N
AU  - Luft,J R
AU  - Pangborn,W
AU  - Chunduru,S K
AU  - Spencer,H T
AU  - Appleman,J R
AU  - Blakley,R L
TI  - Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markers
SN  - 0021-9258
PY  - 1995///0414
KW  - Amino Acid Sequence
KW  - Binding Sites
KW  - Crystallography, X-Ray
KW  - Drug Resistance
KW  - Enzyme Stability
KW  - Genetic Variation
KW  - Humans
KW  - Hydrogen-Ion Concentration
KW  - Kinetics
KW  - Leucine
KW  - Methotrexate
KW  - pharmacology
KW  - Models, Molecular
KW  - Mutagenesis, Site-Directed
KW  - NADP
KW  - metabolism
KW  - Point Mutation
KW  - Protein Conformation
KW  - Recombinant Proteins
KW  - chemistry
KW  - Tetrahydrofolate Dehydrogenase
N1  - Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S
UR  - https://doi.org/10.1074/jbc.270.10.5057
ER  -