Lewis, W S <br/><br/>
Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markers.  [electronic resource] 

 -  The Journal of biological chemistry  Mar 1995 
 - 5057-64 p.  digital 
<br/><br/> Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. 
<br/><br/><label>ISSN: </label>0021-9258 
<br/><br/><label>Standard No.: </label>10.1074/jbc.270.10.5057  doi 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Amino Acid Sequence<br/>Binding Sites<br/>Crystallography, X-Ray<br/>Drug Resistance<br/>Enzyme Stability<br/>Genetic Variation<br/>Humans<br/>Hydrogen-Ion Concentration<br/>Kinetics<br/>Leucine<br/>Methotrexate--pharmacology<br/>Models, Molecular<br/>Mutagenesis, Site-Directed<br/>NADP--metabolism<br/>Point Mutation<br/>Protein Conformation<br/>Recombinant Proteins--chemistry<br/>Tetrahydrofolate Dehydrogenase--chemistry