Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. [electronic resource]
- Nucleic acids research 11 2018
- 10272-10285 p. digital
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
1362-4962
10.1093/nar/gky815 doi
Animals Base Sequence Binding Sites--genetics CRISPR-Associated Proteins--metabolism CRISPR-Cas Systems--genetics Clustered Regularly Interspaced Short Palindromic Repeats Endonucleases--metabolism Francisella--enzymology HEK293 Cells Humans Mammals Mice Moraxella--enzymology Protein Binding Substrate Specificity Tumor Cells, Cultured