Brennan, Jennifer C

Development of a dual luciferase activity and fluorescamine protein assay adapted to a 384 micro-well plate format: Reducing variability in human luciferase transactivation cell lines aimed at endocrine active substances. [electronic resource] - Toxicology in vitro : an international journal published in association with BIBRA Mar 2018 - 18-25 p. digital

Publication Type: Journal Article

1879-3177

10.1016/j.tiv.2017.10.030 doi


Antineoplastic Agents--pharmacology
Biological Assay
Breast Neoplasms--chemically induced
Cell Line, Tumor
Cell-Free System--drug effects
Endocrine Disruptors--toxicity
Female
Fluorescamine--analysis
Fluorescent Dyes--analysis
Gene Expression Regulation, Neoplastic--drug effects
Genes, Reporter--drug effects
High-Throughput Screening Assays
Hormone Antagonists--pharmacology
Humans
Kinetics
Limit of Detection
Luciferases--genetics
Recombinant Proteins--metabolism
Reproducibility of Results
Transcriptional Activation--drug effects