A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid. [electronic resource]
- DNA research : an international journal for rapid publication of reports on genes and genomes Dec 2017
- 609-621 p. digital
Publication Type: Journal Article
1756-1663
10.1093/dnares/dsx029 doi
Animals CRISPR-Cas Systems DNA End-Joining Repair Gene Editing--methods Genomics Green Fluorescent Proteins--genetics HEK293 Cells HeLa Cells Humans Mice NIH 3T3 Cells Plasmids--genetics RNA, Guide, CRISPR-Cas Systems--genetics