TY - GEN AU - Jacobi,Ashley M AU - Rettig,Garrett R AU - Turk,Rolf AU - Collingwood,Michael A AU - Zeiner,Sarah A AU - Quadros,Rolen M AU - Harms,Donald W AU - Bonthuis,Paul J AU - Gregg,Christopher AU - Ohtsuka,Masato AU - Gurumurthy,Channabasavaiah B AU - Behlke,Mark A TI - Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes SN - 1095-9130 PY - 2018///0322 KW - Animals KW - Bacterial Proteins KW - genetics KW - Base Sequence KW - CRISPR-Associated Protein 9 KW - CRISPR-Cas Systems KW - Clustered Regularly Interspaced Short Palindromic Repeats KW - DNA KW - Electroporation KW - Endonucleases KW - Gene Editing KW - methods KW - Gene Targeting KW - Gene Transfer Techniques KW - Genome KW - HEK293 Cells KW - Humans KW - Jurkat Cells KW - Lipids KW - chemistry KW - Mice KW - Microinjections KW - RNA, Guide, CRISPR-Cas Systems KW - chemical synthesis KW - Recombinant Proteins KW - Recombinational DNA Repair KW - Ribonucleoproteins KW - Zygote KW - cytology N1 - Publication Type: Journal Article UR - https://doi.org/10.1016/j.ymeth.2017.03.021 ER -