Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39. [electronic resource]
- Stem cell research Mar 2016
- 308-10 p. digital
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
1876-7753
10.1016/j.scr.2015.12.037 doi
Base Sequence Blotting, Western CRISPR-Cas Systems--genetics Cell Differentiation Cell Line Comparative Genomic Hybridization Embryo, Mammalian--cytology Embryoid Bodies--cytology Exons Female Gene Knockout Techniques Genetic Loci Heterozygote Human Embryonic Stem Cells--cytology Humans Karyotype Microscopy, Fluorescence Neoplasm Proteins--genetics Real-Time Polymerase Chain Reaction Transcription Factors--genetics