TY - GEN AU - Ortiz-Soto,Maria Elena AU - Seibel,Jürgen TI - Expression of Functional Human Sialyltransferases ST3Gal1 and ST6Gal1 in Escherichia coli SN - 1932-6203 PY - 2017///0706 KW - Amino Acid Substitution KW - Antigens, CD KW - chemistry KW - Binding Sites KW - Cloning, Molecular KW - Escherichia coli KW - genetics KW - Gene Expression KW - Glucosides KW - biosynthesis KW - Humans KW - Hydrophobic and Hydrophilic Interactions KW - Kinetics KW - Maltose-Binding Proteins KW - Models, Molecular KW - Molecular Chaperones KW - Mutation KW - Oxidoreductases KW - Protein Binding KW - Protein Disulfide-Isomerases KW - Protein Engineering KW - Protein Folding KW - Protein Interaction Domains and Motifs KW - Protein Structure, Secondary KW - Recombinant Fusion Proteins KW - Sialyltransferases KW - Solubility KW - Substrate Specificity KW - beta-Galactoside alpha-2,3-Sialyltransferase N1 - Publication Type: Journal Article UR - https://doi.org/10.1371/journal.pone.0155410 ER -