TY  - GEN
AU  - Loo,Tip W
AU  - Clarke,David M
TI  - Cysteines introduced into extracellular loops 1 and 4 of human P-glycoprotein that are close only in the open conformation spontaneously form a disulfide bond that inhibits drug efflux and ATPase activity
SN  - 1083-351X
PY  - 2015///0128
KW  - ATP Binding Cassette Transporter, Subfamily B
KW  - chemistry
KW  - Adenosine Triphosphatases
KW  - metabolism
KW  - Adenosine Triphosphate
KW  - Animals
KW  - Binding Sites
KW  - genetics
KW  - Cell Line
KW  - Cysteine
KW  - Disulfides
KW  - Dithiothreitol
KW  - pharmacology
KW  - Drug Resistance, Multiple
KW  - drug effects
KW  - Glycoside Hydrolases
KW  - HEK293 Cells
KW  - Humans
KW  - Hydrolysis
KW  - Immunoblotting
KW  - Models, Molecular
KW  - Mutation
KW  - Pharmaceutical Preparations
KW  - Protein Conformation
KW  - Protein Structure, Secondary
KW  - Protein Structure, Tertiary
N1  - Publication Type: Journal Article; Research Support, Non-U.S. Gov't
UR  - https://doi.org/10.1074/jbc.M114.583021
ER  -