TY - GEN AU - Seeber,Stefan AU - Ros,Francesca AU - Thorey,Irmgard AU - Tiefenthaler,Georg AU - Kaluza,Klaus AU - Lifke,Valeria AU - Fischer,Jens André Alexander AU - Klostermann,Stefan AU - Endl,Josef AU - Kopetzki,Erhard AU - Pashine,Achal AU - Siewe,Basile AU - Kaluza,Brigitte AU - Platzer,Josef AU - Offner,Sonja TI - A robust high throughput platform to generate functional recombinant monoclonal antibodies using rabbit B cells from peripheral blood SN - 1932-6203 PY - 2014///1222 KW - Amino Acid Sequence KW - Animals KW - Antibodies, Monoclonal KW - biosynthesis KW - Antibody Specificity KW - immunology KW - B-Lymphocytes KW - metabolism KW - Cell Separation KW - Cells, Cultured KW - Clone Cells KW - Epitopes KW - HEK293 Cells KW - High-Throughput Screening Assays KW - methods KW - Humans KW - Immunization KW - Immunoglobulin Heavy Chains KW - Immunoglobulin Light Chains KW - Immunoglobulin Variable Region KW - Interleukin-1 Receptor-Like 1 Protein KW - Interleukin-33 KW - Interleukins KW - Protein Binding KW - Rabbits KW - Receptors, Cell Surface KW - Recombinant Proteins N1 - Publication Type: Journal Article; Research Support, Non-U.S. Gov't UR - https://doi.org/10.1371/journal.pone.0086184 ER -