TY - GEN AU - Imamura,Morikazu AU - Kato,Nobuko AU - Okada,Hiroyuki AU - Yoshioka,Miyako AU - Iwamaru,Yoshifumi AU - Shimizu,Yoshihisa AU - Mohri,Shirou AU - Yokoyama,Takashi AU - Murayama,Yuichi TI - Insect cell-derived cofactors become fully functional after proteinase K and heat treatment for high-fidelity amplification of glycosylphosphatidylinositol-anchored recombinant scrapie and BSE prion proteins SN - 1932-6203 PY - 2014///1008 KW - Animals KW - Baculoviridae KW - genetics KW - Biological Assay KW - Blotting, Western KW - Endopeptidase K KW - metabolism KW - Glycosylphosphatidylinositols KW - chemistry KW - Hot Temperature KW - Polymerase Chain Reaction KW - PrPC Proteins KW - PrPSc Proteins KW - Prions KW - Recombinant Proteins N1 - Publication Type: Journal Article; Research Support, Non-U.S. Gov't UR - https://doi.org/10.1371/journal.pone.0082538 ER -