Imamura, Morikazu <br/><br/>
Insect cell-derived cofactors become fully functional after proteinase K and heat treatment for high-fidelity amplification of glycosylphosphatidylinositol-anchored recombinant scrapie and BSE prion proteins.  [electronic resource] 

 -  PloS one  2013 
 - e82538 p.  digital 
<br/><br/> Publication Type: Journal Article; Research Support, Non-U.S. Gov't 
<br/><br/><label>ISSN: </label>1932-6203 
<br/><br/><label>Standard No.: </label>10.1371/journal.pone.0082538  doi 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Animals<br/>Baculoviridae--genetics<br/>Biological Assay<br/>Blotting, Western<br/>Endopeptidase K--metabolism<br/>Glycosylphosphatidylinositols--chemistry<br/>Hot Temperature<br/>Polymerase Chain Reaction<br/>PrPC Proteins--metabolism<br/>PrPSc Proteins--metabolism<br/>Prions--metabolism<br/>Recombinant Proteins--genetics