TY  - GEN
AU  - Weber,Brandon W
AU  - Kimani,Serah W
AU  - Varsani,Arvind
AU  - Cowan,Donald A
AU  - Hunter,Roger
AU  - Venter,Gerhard A
AU  - Gumbart,James C
AU  - Sewell,B Trevor
TI  - The mechanism of the amidases: mutating the glutamate adjacent to the catalytic triad inactivates the enzyme due to substrate mispositioning
SN  - 1083-351X
PY  - 2013///1203
KW  - Acrylamide
KW  - metabolism
KW  - Amidohydrolases
KW  - chemistry
KW  - Biocatalysis
KW  - Catalytic Domain
KW  - Crystallography, X-Ray
KW  - Cysteine
KW  - Enzyme Activation
KW  - Enzyme Stability
KW  - Geobacillus
KW  - enzymology
KW  - Glutamic Acid
KW  - genetics
KW  - Mass Spectrometry
KW  - Mutant Proteins
KW  - Mutation
KW  - Oxidation-Reduction
KW  - Substrate Specificity
N1  - Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
UR  - https://doi.org/10.1074/jbc.M113.503284
ER  -