TY - GEN AU - Ng,Say Kong AU - Tan,Tessa Rui Min AU - Wang,Yang AU - Ng,Daniel AU - Goh,Lin-Tang AU - Bardor,Muriel AU - Wong,Victor Vai Tak AU - Lam,Kong Peng TI - Production of functional soluble Dectin-1 glycoprotein using an IRES-linked destabilized-dihydrofolate reductase expression vector SN - 1932-6203 PY - 2013///0627 KW - Amino Acid Sequence KW - Animals KW - Antifungal Agents KW - chemistry KW - Bioreactors KW - CHO Cells KW - Cloning, Molecular KW - Cricetinae KW - Genetic Vectors KW - Glycoproteins KW - biosynthesis KW - Lectins, C-Type KW - Methotrexate KW - pharmacology KW - Mice KW - Molecular Sequence Data KW - Peptide Chain Initiation, Translational KW - Peptide Fragments KW - Plasmids KW - genetics KW - Protein Binding KW - Protein Engineering KW - Recombinant Fusion Proteins KW - Saccharomyces cerevisiae KW - metabolism KW - Solubility KW - Tetrahydrofolate Dehydrogenase KW - Transcriptional Activation KW - drug effects KW - Zymosan N1 - Publication Type: Journal Article; Research Support, Non-U.S. Gov't UR - https://doi.org/10.1371/journal.pone.0052785 ER -