Identifying novel protein complexes in cancer cells using epitope-tagging of endogenous human genes and affinity-purification mass spectrometry. [electronic resource]
- Journal of proteome research Dec 2012
- 5630-41 p. digital
Publication Type: Journal Article; Research Support, N.I.H., Extramural
1535-3907
10.1021/pr300598t doi
Adaptor Proteins, Signal Transducing--isolation & purification Cell Nucleus--metabolism Chromatography, Affinity--methods Colonic Neoplasms--metabolism Cytoplasm--metabolism Dependovirus Epitope Mapping--methods Genetic Vectors Genome, Human HCT116 Cells HEK293 Cells Humans Mass Spectrometry--methods Microfilament Proteins--isolation & purification Multiprotein Complexes--isolation & purification Neoplasm Proteins--isolation & purification Protein Interaction Mapping--methods Proteomics--methods Receptors, Cytoplasmic and Nuclear--isolation & purification Reproducibility of Results Sensitivity and Specificity Trans-Activators Transfection