Tsuchida, Shirou Hydratase activities of green fluorescent protein tagged human multifunctional enzyme type 2 hydratase domain and its variants. [electronic resource] - Journal of oleo science 2012 - 443-50 p. digital Publication Type: Journal Article ISSN: 1347-3352 Standard No.: 10.5650/jos.61.443 doi Subjects--Topical Terms: 17-Hydroxysteroid Dehydrogenases--chemistryChromatography, High Pressure LiquidFluorescenceGreen Fluorescent Proteins--metabolismHumansHydro-Lyases--chemistryHydrogenationMultienzyme Complexes--chemistryMutagenesis, Site-DirectedMutant Proteins--metabolismPeroxisomal Multifunctional Protein-2Plasmids--metabolismProtein Structure, TertiaryRecombinant Fusion Proteins--isolation & purificationReference Standards