TY - GEN AU - Marshall,Christopher B AU - Meiri,David AU - Smith,Matthew J AU - Mazhab-Jafari,Mohammad T AU - Gasmi-Seabrook,Geneviève M C AU - Rottapel,Robert AU - Stambolic,Vuk AU - Ikura,Mitsuhiko TI - Probing the GTPase cycle with real-time NMR: GAP and GEF activities in cell extracts SN - 1095-9130 PY - 2013///0205 KW - Animals KW - Cell Extracts KW - chemistry KW - Dyneins KW - Enzyme Assays KW - Fluorescent Dyes KW - GTP Phosphohydrolases KW - GTPase-Activating Proteins KW - Guanine Nucleotide Exchange Factors KW - Guanosine Triphosphate KW - HEK293 Cells KW - Humans KW - Hydrolysis KW - Magnetic Resonance Spectroscopy KW - Monomeric GTP-Binding Proteins KW - Neuropeptides KW - Ras Homolog Enriched in Brain Protein KW - Recombinant Proteins KW - Signal Transduction KW - Transfection KW - Tuberous Sclerosis Complex 1 Protein KW - Tuberous Sclerosis Complex 2 Protein KW - Tumor Suppressor Proteins N1 - Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S UR - https://doi.org/10.1016/j.ymeth.2012.06.014 ER -