Probing the GTPase cycle with real-time NMR: GAP and GEF activities in cell extracts. [electronic resource]
- Methods (San Diego, Calif.) Aug 2012
- 473-85 p. digital
Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
1095-9130
10.1016/j.ymeth.2012.06.014 doi
Animals Cell Extracts--chemistry Dyneins--chemistry Enzyme Assays Fluorescent Dyes--chemistry GTP Phosphohydrolases--chemistry GTPase-Activating Proteins--chemistry Guanine Nucleotide Exchange Factors--chemistry Guanosine Triphosphate--chemistry HEK293 Cells Humans Hydrolysis Magnetic Resonance Spectroscopy Monomeric GTP-Binding Proteins--chemistry Neuropeptides--chemistry Ras Homolog Enriched in Brain Protein Recombinant Proteins--chemistry Signal Transduction Transfection Tuberous Sclerosis Complex 1 Protein Tuberous Sclerosis Complex 2 Protein Tumor Suppressor Proteins--chemistry