Kumar, Ashu Optimization and efficient purification of recombinant Omp28 protein of Brucella melitensis using Triton X-100 and β-mercaptoethanol. [electronic resource] - Protein expression and purification Jun 2012 - 226-32 p. digital Publication Type: Journal Article ISSN: 1096-0279 Standard No.: 10.1016/j.pep.2012.04.002 doi Subjects--Topical Terms: Bacterial Proteins--chemistryBrucella melitensis--geneticsCloning, MolecularElectrophoresis, Polyacrylamide GelEscherichia coliGenetic VectorsMembrane Proteins--chemistryMercaptoethanol--chemistryOctoxynol--chemistryProtein DenaturationRecombinant Proteins--chemistrySpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization