TY  - GEN
AU  - Sushma,Krishnan
AU  - Vijayalakshmi,Mookambeswaran A
AU  - Krishnan,Venkataraman
AU  - Satheeshkumar,Padikara Kutty
TI  - Cloning, expression, purification and characterization of a single chain variable fragment specific to tumor necrosis factor alpha in Escherichia coli
SN  - 1873-4863
PY  - 2012///0605
KW  - Antibodies, Monoclonal
KW  - genetics
KW  - Apoptosis
KW  - drug effects
KW  - Cell Line, Tumor
KW  - Chromatography, Affinity
KW  - methods
KW  - Cloning, Molecular
KW  - Electrophoresis, Polyacrylamide Gel
KW  - Escherichia coli
KW  - Humans
KW  - Recombinant Proteins
KW  - biosynthesis
KW  - Single-Chain Antibodies
KW  - Sodium Chloride
KW  - Substrate Specificity
KW  - Tumor Necrosis Factor-alpha
KW  - metabolism
N1  - Publication Type: Journal Article; Research Support, Non-U.S. Gov't
UR  - https://doi.org/10.1016/j.jbiotec.2011.06.039
ER  -