Sushma, Krishnan <br/><br/>
Cloning, expression, purification and characterization of a single chain variable fragment specific to tumor necrosis factor alpha in Escherichia coli.  [electronic resource] 

 -  Journal of biotechnology  Dec 2011 
 - 238-44 p.  digital 
<br/><br/> Publication Type: Journal Article; Research Support, Non-U.S. Gov't 
<br/><br/><label>ISSN: </label>1873-4863 
<br/><br/><label>Standard No.: </label>10.1016/j.jbiotec.2011.06.039  doi 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Antibodies, Monoclonal--genetics<br/>Apoptosis--drug effects<br/>Cell Line, Tumor<br/>Chromatography, Affinity--methods<br/>Cloning, Molecular--methods<br/>Electrophoresis, Polyacrylamide Gel<br/>Escherichia coli--genetics<br/>Humans<br/>Recombinant Proteins--biosynthesis<br/>Single-Chain Antibodies--biosynthesis<br/>Sodium Chloride<br/>Substrate Specificity<br/>Tumor Necrosis Factor-alpha--metabolism