TY - GEN AU - Zhang,Hong-Ying AU - Chen,Pei-Fu AU - Xu,Jia-Ming AU - Dai,Quan-Min AU - Xu,Feng AU - Han,Qing-Wang AU - Wang,Jian-Jun AU - Jin,Hei-Ying TI - Separation and purification of Escherichia coli-expressed human thymosin-α1 using affinity chromatography and high-performance liquid chromatography SN - 1096-0279 PY - 2011///0630 KW - Chromatography, Affinity KW - methods KW - Chromatography, High Pressure Liquid KW - Cloning, Molecular KW - Escherichia coli KW - Gene Expression KW - Histidine KW - metabolism KW - Humans KW - Isoelectric Focusing KW - Isopropyl Thiogalactoside KW - Oligopeptides KW - Protein Denaturation KW - Recombinant Fusion Proteins KW - genetics KW - Reproducibility of Results KW - Solubility KW - Thymalfasin KW - Thymosin KW - analogs & derivatives N1 - Publication Type: Journal Article; Research Support, Non-U.S. Gov't UR - https://doi.org/10.1016/j.pep.2011.01.004 ER -