TY - GEN AU - Small,Evan AU - Eggler,Aimee AU - Mesecar,Andrew D TI - Development of an efficient E. coli expression and purification system for a catalytically active, human Cullin3-RINGBox1 protein complex and elucidation of its quaternary structure with Keap1 SN - 1090-2104 PY - 2010///1123 KW - Carrier Proteins KW - biosynthesis KW - Catalysis KW - Chromatography, Affinity KW - Chromatography, Gel KW - Cullin Proteins KW - Escherichia coli KW - genetics KW - Humans KW - Intracellular Signaling Peptides and Proteins KW - chemistry KW - Kelch-Like ECH-Associated Protein 1 KW - Protein Structure, Quaternary KW - Ubiquitination KW - Ultracentrifugation N1 - Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't UR - https://doi.org/10.1016/j.bbrc.2010.08.062 ER -