Development of an efficient E. coli expression and purification system for a catalytically active, human Cullin3-RINGBox1 protein complex and elucidation of its quaternary structure with Keap1. [electronic resource]
- Biochemical and biophysical research communications Oct 2010
- 471-5 p. digital
Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
1090-2104
10.1016/j.bbrc.2010.08.062 doi
Carrier Proteins--biosynthesis Catalysis Chromatography, Affinity Chromatography, Gel Cullin Proteins--biosynthesis Escherichia coli--genetics Humans Intracellular Signaling Peptides and Proteins--chemistry Kelch-Like ECH-Associated Protein 1 Protein Structure, Quaternary Ubiquitination Ultracentrifugation