Development of high-throughput TR-FRET and AlphaScreen assays for identification of potent inhibitors of PDK1. [electronic resource]
- Journal of biomolecular screening Dec 2009
- 1257-62 p. digital
Publication Type: Journal Article
1552-454X
10.1177/1087057109349356 doi
3-Phosphoinositide-Dependent Protein Kinases Adenosine Triphosphate--metabolism Biotinylation--drug effects Fluorescence Resonance Energy Transfer--methods High-Throughput Screening Assays--methods Humans Kinetics Peptides--metabolism Protein Kinase Inhibitors--analysis Protein Serine-Threonine Kinases--antagonists & inhibitors Proto-Oncogene Proteins c-akt--metabolism Reproducibility of Results Signal Transduction--drug effects Structure-Activity Relationship Substrate Specificity--drug effects Time Factors