TY  - GEN
AU  - Thomas,James L
AU  - Boswell,Elizabeth L
AU  - Scaccia,Launa A
AU  - Pletnev,Vladimir
AU  - Umland,Timothy C
TI  - Identification of key amino acids responsible for the substantially higher affinities of human type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD1) for substrates, coenzymes, and inhibitors relative to human 3beta-HSD2
SN  - 0021-9258
PY  - 2005///0712
KW  - 3-Hydroxysteroid Dehydrogenases
KW  - chemistry
KW  - Allosteric Site
KW  - Amino Acid Sequence
KW  - Androstenols
KW  - Animals
KW  - Arginine
KW  - Baculoviridae
KW  - metabolism
KW  - Blotting, Western
KW  - Catalysis
KW  - Catalytic Domain
KW  - Cell Line
KW  - DNA Primers
KW  - Dihydrotestosterone
KW  - analogs & derivatives
KW  - Dose-Response Relationship, Drug
KW  - Electrophoresis, Polyacrylamide Gel
KW  - Glutamine
KW  - Histidine
KW  - Humans
KW  - Insecta
KW  - Kinetics
KW  - Models, Chemical
KW  - Models, Molecular
KW  - Molecular Sequence Data
KW  - Mutagenesis, Site-Directed
KW  - Mutation
KW  - NAD
KW  - Protein Binding
KW  - Protein Conformation
KW  - Sequence Homology, Amino Acid
KW  - Structure-Activity Relationship
KW  - Substrate Specificity
KW  - Tissue Distribution
N1  - Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S
UR  - https://doi.org/10.1074/jbc.M501269200
ER  -