TY - GEN AU - Traggiai,Elisabetta AU - Becker,Stephan AU - Subbarao,Kanta AU - Kolesnikova,Larissa AU - Uematsu,Yasushi AU - Gismondo,Maria Rita AU - Murphy,Brian R AU - Rappuoli,Rino AU - Lanzavecchia,Antonio TI - An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus SN - 1078-8956 PY - 2004///1004 KW - Adult KW - Animals KW - Antibodies, Monoclonal KW - isolation & purification KW - B-Lymphocytes KW - immunology KW - Chlorocebus aethiops KW - DNA Primers KW - Disease Models, Animal KW - Enzyme-Linked Immunosorbent Assay KW - Herpesvirus 4, Human KW - metabolism KW - Humans KW - Immunization, Passive KW - methods KW - Immunologic Memory KW - Membrane Glycoproteins KW - genetics KW - Mice KW - Microscopy, Immunoelectron KW - Neutralization Tests KW - Polymerase Chain Reaction KW - Severe Acute Respiratory Syndrome KW - Spike Glycoprotein, Coronavirus KW - Transformation, Genetic KW - Vero Cells KW - Viral Envelope Proteins KW - alpha-Macroglobulins N1 - Publication Type: Journal Article; Research Support, Non-U.S. Gov't UR - https://doi.org/10.1038/nm1080 ER -