TY - GEN AU - Chao,Yun-Peng AU - Chern,Jong-Tzer AU - Lin,Wei Shing AU - Wang,Zei Wen TI - Development of a fed-batch fermentation process to overproduce phosphoenolpyruvate carboxykinase using an expression vector with promoter and plasmid copy number controllable by heat SN - 0006-3592 PY - 2004///0720 KW - Bacteriophage T7 KW - genetics KW - Bioreactors KW - microbiology KW - Cell Culture Techniques KW - methods KW - Cell Division KW - radiation effects KW - Escherichia coli KW - cytology KW - Feasibility Studies KW - Fermentation KW - physiology KW - Gene Dosage KW - Gene Expression Regulation, Bacterial KW - Gene Expression Regulation, Enzymologic KW - Heat-Shock Response KW - Hot Temperature KW - Isopropyl Thiogalactoside KW - Phosphoenolpyruvate Carboxykinase (ATP) KW - biosynthesis KW - Plasmids KW - Promoter Regions, Genetic KW - Protein Engineering KW - Recombinant Fusion Proteins KW - Transfection N1 - Publication Type: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't UR - https://doi.org/10.1002/bit.10796 ER -