Chao, Yun-Peng <br/><br/>
Development of a fed-batch fermentation process to overproduce phosphoenolpyruvate carboxykinase using an expression vector with promoter and plasmid copy number controllable by heat.  [electronic resource] 

 -  Biotechnology and bioengineering  Nov 2003 
 - 459-66 p.  digital 
<br/><br/> Publication Type: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't 
<br/><br/><label>ISSN: </label>0006-3592 
<br/><br/><label>Standard No.: </label>10.1002/bit.10796  doi 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Bacteriophage T7--genetics<br/>Bioreactors--microbiology<br/>Cell Culture Techniques--methods<br/>Cell Division--radiation effects<br/>Escherichia coli--cytology<br/>Feasibility Studies<br/>Fermentation--physiology<br/>Gene Dosage<br/>Gene Expression Regulation, Bacterial--radiation effects<br/>Gene Expression Regulation, Enzymologic--radiation effects<br/>Heat-Shock Response--physiology<br/>Hot Temperature<br/>Isopropyl Thiogalactoside--genetics<br/>Phosphoenolpyruvate Carboxykinase (ATP)--biosynthesis<br/>Plasmids--genetics<br/>Promoter Regions, Genetic<br/>Protein Engineering--methods<br/>Recombinant Fusion Proteins--biosynthesis<br/>Transfection--methods